NHS-PEG5-bis-PEG3-azide combines an amine-reactive NHS ester, a hydrophilic PEG5 spacer, and two PEG3-linked azide groups in a multifunctional linker and specifically supports amine labeling, click chemistry, branched bioconjugation, and multicomponent molecular assembly.
Key Features and Benefits
- Multifunctional Linker: Combines one NHS ester with two azide functionalities for sequential and branched conjugation strategies.
- NHS Ester Functionality: Reacts with primary amines on proteins, peptides, and other amine-containing molecules.
- PEG5 Spacer: Provides a flexible, hydrophilic spacer between the NHS-reactive region and the branched linker structure.
- Dual PEG3-Azide Groups: Two PEG3-linked azides specifically provide separate reactive sites for click-based functionalization.
- CuAAC Click Chemistry: Terminal azides react with suitable alkynes specifically through copper-catalyzed azide-alkyne cycloaddition.
- SPAAC Click Chemistry: Azide groups react with strained cyclooctynes such as DBCO and BCN through copper-free click chemistry.
- Stable Triazole Formation: Azide-alkyne reactions specifically generate stable triazole linkages for constructing molecular conjugates.
- Sequential Functionalization: NHS chemistry can first modify an amine-bearing molecule, leaving two azides available for subsequent click reactions.
Applications
- Bioconjugation: Supports multistep attachment of compatible biomolecules, probes, ligands, and other functional components.
- Amine Labeling: NHS ester functionality enables modification of primary amines on suitable biomolecules and synthetic compounds.
- Click Chemistry: Dual azide groups support CuAAC and SPAAC reactions with compatible click partners.
- Branched Conjugation: Two azide functionalities enable attachment of multiple compatible molecules through a single linker architecture.
- Protein Modification: Useful for introducing dual azide functionality onto proteins containing accessible primary amines.
- Peptide Modification: Supports amine-directed functionalization of peptides for subsequent click-based conjugation.
- Nanoparticle Functionalization: Enables multivalent modification of appropriately functionalized nanoparticle systems.
- Biomaterial Research: Supports construction of functional materials using amine-reactive and click-compatible conjugation strategies.
Storage and Handling
- Storage: Store according to the product specification in a tightly sealed container under dry conditions.
- Handling: NHS esters are moisture sensitive and can hydrolyze in aqueous environments. Minimize moisture exposure and prepare aqueous solutions shortly before use.
NHS-PEG5-bis-PEG3-azide combines amine-reactive NHS chemistry with two click-reactive azide groups, while its hydrophilic PEG architecture supports sequential conjugation, branched functionalization, and multicomponent molecular design.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
