Filling the performance gap between fast‑eroding 2K and medium‑molecular‑weight 5K PCL‑N3, this 3 kDa azide‑capped poly(ε‑caprolactone) delivers a practical balance of chain crystallinity, solvent compatibility and degradation timeline. Its terminal azide group enables efficient copper‑catalyzed azide‑alkyne cycloaddition (CuAAC), forming stable triazole linkages when coupled with alkyne‑modified peptides, small‑molecule ligands or surface substrates. Compared with MW 2K, extended chain length slows hydrolysis rate and improves colloidal particle stability after nanoprecipitation, extending usable experimental windows from short‑term acute assays toward multi‑week in‑vitro testing. This reactive hydrophobic polyester is widely adopted for building click‑assembled nanoparticle libraries and tuning surface ligand densities, ideal for intermediate‑duration cell‑based research and preliminary small‑scale in‑vivo exploration.
Key Features: ‑ Intermediate‑length PCL backbone equipped with terminal azide functionality, well‑adapted for CuAAC click reactions under mixed organic‑aqueous solvent environments ‑ Moderately increased crystallinity relative to 2 K counterpart; still maintains good solubility in DCM, chloroform and THF while generating more dimension‑stable colloidal particles ‑ Intermediate hydrolytic erosion kinetics, sustaining structural integrity over multi‑week experimental cycles before gradual mass loss occurs ‑ Chemically inert to native thiols, amines and hydroxyl groups within biological systems; reaction specificity is strictly restricted to alkyne‑containing click partners ‑ Spectroscopically confirmed azide end‑group conversion; controlled residual impurity levels suitable for cell‑culture screening and preliminary biological prototype evaluation
Applications: ‑ Intermediate‑persistence azide‑labelled micelles and nanoparticles for multi‑week cellular uptake profiling and drug‑release behaviour assessment ‑ Click‑driven surface modification of polymer substrates to anchor alkyne‑functionalized peptides for intermediate‑term cell phenotype studies ‑ Assembly of polymer‑biomolecule triazole‑linked conjugates for mid‑duration biological observation, sitting between transient 2K constructs and higher‑MW long‑residing analogues ‑ Bench‑top formulation screening to connect fast‑degrading low‑MW and mechanically robust medium‑MW PCL‑N3 material series ‑ Exploratory development of click‑functionalized thin polymer coatings for in‑vitro biomimetic culture platforms
Handling & Storage: Store lyophilized polymer powder at −20 °C under dry inert‑gas conditions; avoid persistent humid surroundings to preserve azide end‑group integrity. Though azide moieties possess better hydrolytic stability compared with maleimide, long‑term moisture intrusion will still compromise click‑reaction performance. Allow sealed sample vials to fully equilibrate inside a desiccator before opening, preventing water condensation from contaminating solid samples. Prepare experimental working solutions with anhydrous organic solvents immediately before use; long‑term preservation of dissolved polymer stock is discouraged. This product is for laboratory‑only research and has not been approved for human clinical applications.
Product Short Description:
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.