MGB CPG 1000
MGB CPG 1000 is a solid support used in oligonucleotide synthesis to attach a Minor Groove Binder (MGB) at the 3′-end of oligonucleotides. The MGB increases binding affinity and stability by interacting with the minor groove of the DNA helix, enhancing specificity in molecular diagnostics.
Key Features:
- Pore Size: 1000 Å (supports longer oligonucleotide synthesis)
- Loading Capacity: >15 µmol/g
- Compatibility: Works with standard synthesis protocols, including UltraMild monomers.
- Oxidation Tip: Use 0.5 M CSO in acetonitrile for better results.
MGB CPG 1000 Applications:
- Enhanced Hybridization Probes:
- Improves melting temperature (Tm) and stability.
- Useful for designing shorter, more specific oligonucleotide probes.
- Commonly used in quantitative PCR assays like TaqMan MGB probes.
Handling and Storage:
- Appearance: Off-white beads
- Storage: Store at -20°C in the dark (stable for 24 months).
- Transportation: Stable at room temperature for 3 weeks if kept dry.
MGB CPG is ideal for applications requiring high-binding affinity, such as molecular diagnostics, gene expression analysis, and mutation detection.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
