Methyltetrazine-PEG-amine linker has a table methyltetrazine on one end and a primary amine on the other. The methyltetrazine provide an ideal biorthogonal for TCO conjugated molecules. The primary amine group on the other end opens many options for conjugation with active esters such as NHS and PFP.
The PEG spacer installed increases the solubility of the bioconjugates and potentially minimizes the immunogenicity and improves pharmacokinetics. The short PEG chain has shown to reduce aggregation of ADCs and prevent fluorophore quenching.
AxisPharm provide customized synthesis of tetrazine PEG linkers with various functional groups in high quality.