DSPE-Alkyne

Key Properties

• A functionalized phospholipid consisting of a DSPE lipid backbone and a terminal alkyne (-C≡CH) group on the hydrophilic head.
• Amphiphilic structure with highly hydrophobic saturated stearoyl tails, providing excellent stability in lipid bilayers.
• Reactive alkyne group enables bioorthogonal click chemistry, specifically copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-modified molecules.
• Can also be used in strain-promoted cycloaddition with DBCO-containing compounds when copper-free conditions are required.
• High biocompatibility and low toxicity, suitable for biological systems.
• Insoluble in water but soluble in organic solvents such as chloroform, methanol, and DMSO.
• Typically appears as a white to off-white waxy solid or powder.
• Stable under inert atmosphere; recommended storage at −20 °C, protected from light and moisture.

Applications

• Surface modification of liposomes, lipid nanoparticles (LNPs), micelles and exosomes via click chemistry.
• Conjugation with azide-functionalized targeting ligands, peptides, antibodies, and aptamers for targeted drug delivery.
• Labeling of lipid assemblies with fluorescent or radioactive azide probes for in vitro and in vivo imaging.
• Construction of multifunctional theranostic nanoparticles combining therapy, targeting, and imaging.
• Preparation of functional PEGylated lipids and biomimetic membrane systems.
• Used in nucleic acid delivery systems (DNA, mRNA, siRNA) for surface functionalization of delivery vehicles.