APDye Fluor 488 Tyramide is a green fluorescent dye used in Tyramide Signal Amplification (TSA) for detecting low-abundance targets in applications like immunohistochemistry (IHC), immunocytochemistry (ICC), and in situ hybridization (ISH). It enhances signal intensity, providing high sensitivity and resolution in fluorescence imaging.
Key Features:
- Excitation/Emission Maxima: ~495 nm / ~519 nm (green fluorescence)
- Detection: Compatible with FITC filter sets
- Applications: Ideal for IHC, ICC, ISH, and multiplex fluorescence imaging
How It Works:
- Sample Preparation: Fix and permeabilize cells or tissue samples.
- Primary Antibody Binding: Apply a primary antibody specific to the target antigen.
- HRP-Conjugated Secondary Antibody: Use a secondary antibody with horseradish peroxidase (HRP).
- Tyramide Reaction: Apply APDye Fluor 488 Tyramide; HRP catalyzes its deposition, creating localized fluorescence.
Advantages:
- Enhanced Sensitivity: Amplifies signals for low-abundance targets.
- High Resolution: Localizes fluorescence with minimal background.
- Multiplexing: Enables simultaneous detection of multiple targets with different dyes.
Storage & Handling:
- Storage Conditions: 2°C to 8°C, protected from light
- Precautions: Avoid repeated freeze-thaw cycles.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
