APDye 488 TCO, an AF488 or Alexa Fluor 488 equivalent, is powerful fluorescent dyes for bioconjugation and imaging. Both feature TCO (Trans-cyclooctene), which reacts quickly with tetrazines through inverse electron-demand Diels-Alder cycloaddition (IEDDA). This reaction is fast, selective, and stable in water, making it perfect for low-concentration tasks like protein-protein conjugation.
Key Features:
- APDye 488 TCO: Identical to AF488 or Alexa 488, a bright and hydrophilic fluorophore. It absorbs at 495 nm and emits at 519 nm. APDye488 TCOI is ideal for green-channel imaging and offers excellent performance in bioorthogonal chemistry and green fluorescence.
Applications:
- Protein Labeling: Targets proteins with high precision in low-concentration conditions.
- Live-Cell Imaging: Visualizes biomolecules effectively in living cells.
- Fast Bioorthogonal Reactions: Ensures quick and selective reactions for both in vivo and in vitro studies.
- Fluorescence Imaging: Works well in flow cytometry and microscopy with its bright, stable emission.
These dyes enhance bioconjugation tasks with their speed, selectivity, and strong fluorescence.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
