2′-OMe-Ac-C Phosphoramidite provides a protected 2′-O-methylcytidine building block for RNA oligonucleotide synthesis and nucleic acid modification research and specifically combines a 2′-O-methyl ribose modification with an acetyl-protected cytosine nucleobase and reactive phosphoramidite functionality. This modified nucleoside specifically supports synthesis of RNA analogs, antisense oligonucleotides, and chemically modified nucleic acid sequences.
Key Features and Benefits
- 2′-O-Methyl Modification: Features a methyl group at the ribose 2′-oxygen, specifically supporting enhanced nuclease resistance and modified RNA duplex properties.
- Acetyl-Protected Cytosine: Contains an acetyl protecting group on the cytosine exocyclic amino functionality to minimize unwanted reactions during synthesis.
- Reactive Phosphoramidite Functionality: Enables incorporation into growing oligonucleotide chains using compatible solid-phase phosphoramidite coupling protocols.
- RNA-Compatible Nucleoside: Provides a chemically modified cytidine building block for preparing RNA analogs and mixed-modification oligonucleotides.
- Improved Nuclease Resistance: Supports development of oligonucleotides with increased resistance to enzymatic degradation compared with corresponding unmodified RNA sequences.
- Enhanced Hybridization Properties: The 2′-O-methyl modification can improve complementary RNA binding affinity and duplex stability in suitable sequences.
- Controlled Nucleotide Incorporation: Enables sequence-specific introduction of modified cytidine residues specifically during automated oligonucleotide assembly.
- Protecting Group Compatibility: Supports controlled synthesis and subsequent deprotection specifically using conditions appropriate for acetyl-protected nucleobases.
- Versatile Oligonucleotide Modification: Enables investigation of RNA stability, molecular recognition, and nucleic acid structure-function relationships.
Applications
- Modified RNA Synthesis: Supports preparation of RNA oligonucleotides containing 2′-O-methylcytidine residues at selected positions.
- Antisense Oligonucleotide Research: Enables development of modified antisense sequences for investigating RNA recognition and target binding.
- siRNA Modification Research: Supports incorporation of 2′-O-methylcytidine into small interfering RNA strands for stability and activity studies.
- RNA Hybridization Studies: Especially useful for investigating duplex formation, melting temperature, and sequence-specific molecular recognition.
- Nuclease Resistance Studies: Enables evaluation of 2′-O-methyl modifications on oligonucleotide degradation and stability.
- RNA Probe Development: Supports synthesis of modified RNA probes for hybridization and molecular detection research.
- Aptamer Research: Useful for preparing chemically modified RNA aptamers and investigating their binding properties.
- RNA Structure Studies: Supports investigation of ribose modification effects on RNA conformation, folding, and molecular interactions.
- Phosphoramidite Chemistry Development: Enables optimization of coupling, deprotection, and purification procedures for modified RNA building blocks.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container under an inert atmosphere when possible, unless otherwise specified. Protect from moisture, excessive heat, and prolonged light exposure.
- Handling: Handle under anhydrous conditions to preserve phosphoramidite reactivity. Prepare solutions using dry, compatible solvents, such as anhydrous acetonitrile, when appropriate. Minimize exposure to atmospheric moisture and use freshly prepared solutions for oligonucleotide synthesis. Select coupling and deprotection conditions compatible with the acetyl protecting group and 2′-O-methyl modification.
2′-OMe-Ac-C Phosphoramidite combines a 2′-O-methyl ribose modification with acetyl-protected cytosine for controlled oligonucleotide synthesis and specifically supports modified RNA development, antisense research, siRNA modification, nuclease stability studies, and advanced nucleic acid chemistry.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
