SPDP-PEG-t-Butyl ester contains a PEG chain with a t-butyl ester functional group at one end and a pyridyldithiol reactive group at the other end. The t-butyl ester group is known for its hydrophobicity, while the pyridyldithiol group is known for its ability to react with sulfhydryl groups (-SH) in proteins or other molecules, allowing for the formation of stable covalent linkages.
SPDP-PEG-t-Butyl ester has been widely used in the field of pharmaceutical research and development as a crosslinker for the covalent modification of proteins and other biomolecules. Its unique chemical properties allow for the controlled modification of specific functional groups on target molecules, resulting in improved pharmacological properties such as increased stability, solubility, and bioactivity.
SPDP-PEG-t-Butyl ester has been utilized in the development of targeted drug delivery systems, such as antibody-drug conjugates (ADCs). These systems involve the conjugation of a therapeutic agent to an antibody molecule, which recognizes and binds to specific cancer cells. The PEG component in SPDP-PEG-t-Butyl ester provides steric hindrance and prolongs the circulation time of the conjugate in vivo, while the t-butyl ester group allows for controlled release of the therapeutic agent at the target site through hydrolysis.