α‑Hydroxyl‑ω‑FITC‑poly(D,L‑lactide) (PDLLA‑FITC, MW 5K) is linear, mono‑fluorescein‑isothiocyanate (FITC)‑terminated fully amorphous biodegradable aliphatic polyester. D‑lactide and L‑lactide repeat units are randomly distributed along polymer backbone, disrupting stereoregularity and eliminating crystalline domains. Compared with higher‑molecular‑weight PDLLA‑FITC analogues, this 5 kDa grade displays moderate hydrophobicity and relatively fast, homogeneous hydrolytic degradation. One chain end is covalently conjugated with FITC (green‑emitting fluorescent dye, excitation ~495 nm, emission ~525 nm), while the opposite terminus retains inert hydroxyl (‑OH). The FITC moiety enables direct fluorescence tracking and qualitative/quantitative detection of PDLLA‑based carriers under fluorescence microscopy, flow cytometry and in‑vitro/in‑vivo imaging. It serves as fluorescent tracer macromonomer for nanoparticle visualization, biodistribution research and biodegradation‑monitoring biomedical studies.
Key Features
- MW: 5000 Da, linear α‑hydroxyl‑ω‑FITC‑terminated poly(D,L‑lactide)
- Fully amorphous non‑crystalline structure, random D/L‑lactide sequence, no melting point
- Moderate hydrophobicity, relatively fast and uniform hydrolytic degradation versus higher‑MW PDLLA‑FITC
- Excellent biocompatibility; degradation produces non‑toxic lactic‑acid metabolites
- Single terminal FITC fluorophore; Ex/Em ≈495 nm / 525 nm; opposite polymer end is inert hydroxyl ‑OH
- Narrow molecular‑weight distribution, stable batch‑to‑batch reproducibility; controlled dye‑labeling ratio
- Soluble in DCM, CHCl₃, THF, DMF, acetone; insoluble in water
- FITC fluorophore highly light‑sensitive; strictly avoid prolonged light exposure, high‑temperature and acidic‑alkaline conditions
Applications
- Fluorescent tracer building block for preparing FITC‑labelled PDLLA nanoparticles, microspheres and polymer films
- In‑vitro cell uptake observation, intracellular trafficking visualization by fluorescence microscopy and flow cytometry
- In‑vitro & preliminary in‑vivo biodistribution, polymer degradation and carrier clearance monitoring research
- Research‑grade fluorescent raw material for visualizing controlled‑release drug‑delivery systems
Handling & Storage
- Store at −20 °C under dry inert nitrogen or argon atmosphere; strictly moisture‑proof and full light‑protection
- Keep hermetically sealed; moisture, heat and illumination cause ester hydrolysis and FITC photobleaching; avoid repeated freeze‑thaw cycles
- For research‑only use; not intended for clinical or diagnostic applications
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.