α‑Hydroxyl‑ω‑FITC‑poly(D,L‑lactide) (PDLLA‑FITC, MW 10K) is linear, mono‑fluorescein‑isothiocyanate (FITC)‑terminated fully amorphous biodegradable aliphatic polyester. D‑lactide and L‑lactide repeat units are randomly distributed along polymer backbone, disrupting stereoregularity and eliminating crystalline domains. Compared with MW 5K PDLLA‑FITC, this 10 kDa variant shows higher hydrophobicity and markedly slower yet homogeneous hydrolytic degradation. One chain end is covalently conjugated with FITC green‑emitting fluorescent dye (excitation ~495 nm, emission ~525 nm), while the opposite terminus retains inert hydroxyl (‑OH). The FITC moiety enables direct fluorescence tracing, qualitative and quantitative detection of PDLLA‑based carriers via fluorescence microscopy, flow cytometry and in‑vitro / in‑vivo imaging. It functions as fluorescent tracer macromonomer for nanoparticle visualization, long‑term biodistribution evaluation and biodegradation‑monitoring biomedical research.
Key Features
- MW: 10000 Da, linear α‑hydroxyl‑ω‑FITC‑terminated poly(D,L‑lactide)
- Fully amorphous non‑crystalline structure, random D/L‑lactide sequence, no melting point
- Higher hydrophobicity, slower and uniform hydrolytic degradation versus 5K PDLLA‑FITC
- Excellent biocompatibility; degradation produces non‑toxic lactic‑acid metabolites
- Single terminal FITC fluorophore; Ex/Em ≈495 nm / 525 nm; opposite polymer end is inert hydroxyl ‑OH
- Narrow molecular‑weight distribution, stable batch‑to‑batch reproducibility; controlled dye‑labeling ratio
- Soluble in DCM, CHCl₃, THF, DMF, hot acetone; insoluble in water
- FITC fluorophore highly light‑sensitive; strictly avoid prolonged light exposure, high‑temperature and extreme pH conditions
Applications
- Fluorescent tracer building block for preparing FITC‑labelled PDLLA nanoparticles, microspheres and polymer films for long‑acting systems
- In‑vitro cell uptake observation and intracellular trafficking visualization by fluorescence microscopy and flow cytometry
- In‑vitro & in‑vivo biodistribution, polymer degradation and carrier clearance monitoring for sustained‑release study
- Research‑grade fluorescent raw material for visualizing long‑circulating controlled‑release drug‑delivery systems
Handling & Storage
- Store at −20 °C under dry inert nitrogen or argon atmosphere; strictly moisture‑proof and full light‑protection
- Keep hermetically sealed; moisture, heat and illumination cause ester hydrolysis and FITC photobleaching; avoid repeated freeze‑thaw cycles
- For research‑only use; not intended for clinical or diagnostic applications
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.