Tetrazine-PEG9-Tetrazine is a bifunctional polyethylene glycol (PEG) linker designed for bioorthogonal click chemistry, biomolecular conjugation, and crosslinking applications. It contains two terminal tetrazine groups connected by a flexible, hydrophilic PEG9 spacer. These reactive groups undergo rapid inverse-electron-demand Diels–Alder (IEDDA) reactions with compatible strained alkenes, particularly trans-cyclooctene (TCO). Additionally, its dual functionality enables molecular bridging and the preparation of crosslinked biomolecular structures.
Key Features and Benefits
- Bifunctional Tetrazine Linker: Features two terminal tetrazine groups for conjugation with compatible strained alkene-functionalized molecules.
- Hydrophilic PEG9 Spacer: Contains nine ethylene glycol units that provide flexibility and improve compatibility with aqueous reaction environments.
- Bioorthogonal Click Chemistry: Supports selective IEDDA reactions with TCO and other suitable strained alkene groups.
- Copper-Free Conjugation: Enables covalent coupling without copper catalysts, making it useful for compatible biological labeling systems.
- Rapid Tetrazine–TCO Reaction: Supports efficient conjugation under mild conditions through the high reactivity of tetrazines toward TCO.
- Dual Reactive Functionality: Enables molecular bridging between two compatible strained alkene-containing components.
- Flexible Molecular Spacer: Specifically provides separation between conjugated molecules, helping reduce steric interference in suitable molecular assemblies.
- Crosslinking Capability: Supports formation of covalently connected molecular structures through reactions at both tetrazine termini.
- Versatile Bioconjugation: Additionally, facilitates development of functionalized biomolecules, polymer networks, and bioorthogonal research materials.
Applications
- Bioorthogonal Click Chemistry: Enables selective conjugation with TCO-functionalized molecules through IEDDA reactions.
- Tetrazine–TCO Bioconjugation: Supports covalent attachment of compatible proteins, peptides, polymers, and other functionalized biomolecules.
- Bifunctional Molecular Crosslinking: Facilitates bridging between two strained alkene-containing molecules or molecular structures.
- Protein Conjugation: Supports preparation of protein conjugates when proteins contain suitable TCO or related reactive handles.
- Peptide Conjugation: Specifically enables coupling of TCO-functionalized peptides for molecular assembly and biochemical research.
- Hydrogel Formation: Supports crosslinking of appropriately functionalized polymer systems for hydrogel and biomaterials research.
- PEG-Based Linker Development: Provides a hydrophilic spacer for preparing functionalized molecular assemblies and conjugates.
- Surface Functionalization: Enables modification of TCO-functionalized surfaces and materials through tetrazine click chemistry.
- Biomaterials Research: Additionally, supports development of crosslinked polymer networks, molecular scaffolds, and bioorthogonal materials.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container, protected from light and moisture, unless product-specific documentation indicates otherwise. Additionally, minimize temperature fluctuations to preserve tetrazine reactivity.
- Handling: Use clean, dry equipment and appropriate protective gear. Prepare solutions in a compatible solvent, such as DMSO or an appropriate aqueous buffer, after confirming solubility.
- Click Chemistry Conjugation: React the tetrazine groups with TCO-functionalized molecules under suitable reaction conditions. Subsequently, optimize concentration, reaction time, and stoichiometry to achieve the desired conjugation or crosslinking.
- Bioconjugation and Analysis: Purify conjugates using chromatography or other appropriate methods. Confirm reaction completion and product identity using HPLC, LC-MS, or suitable analytical techniques.
Tetrazine-PEG9-Tetrazine combines dual tetrazine functionality with a flexible PEG9 spacer for efficient bioorthogonal conjugation. Its copper-free IEDDA reactivity supports molecular bridging, while its hydrophilic linker facilitates compatibility with suitable biological and polymer systems. Therefore, it provides a versatile reagent for TCO conjugation, biomolecular crosslinking, hydrogel development, and biomaterials research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
