TCO-PEG5-Biotin
This product is a heterobifunctional linker containing a trans-cyclooctene (TCO) group, a PEG5 spacer, and biotin. It specifically enables rapid tetrazine ligation and high-affinity biotin–streptavidin binding, offering solubility, flexibility, and dual reactivity.
Key Features and Benefits
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TCO Group: Reacts rapidly with tetrazines via IEDDA bioorthogonal click chemistry.
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Biotin Moiety: Provides strong, specific binding to streptavidin or avidin.
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PEG5 Spacer: Enhances solubility and reduces steric hindrance.
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Dual Functionality: Combines bioorthogonal click reactivity with affinity capture.
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High Purity: Ensures reproducibility in conjugation and assay workflows.
Applications
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Bioconjugation: Links tetrazine-modified biomolecules with biotin for affinity detection.
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Probe Development: Builds multifunctional probes for imaging and diagnostics.
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Affinity Capture: Facilitates purification and detection via biotin–streptavidin systems.
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Drug Delivery: Provides PEG scaffolds with orthogonal reactivity and capture handles.
Storage and Handling
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Storage: Store at –20 °C in a sealed, light- and moisture-protected container.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid prolonged exposure to moisture.
This product integrates rapid tetrazine click chemistry with biotin–streptavidin binding in a PEG5 scaffold, specifically enabling versatile conjugation and detection strategies.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
