TCO-PEG4-NHS ester
TCO-PEG4-NHS ester is a high-purity PEG linker with a trans-cyclooctene (TCO) group for rapid, catalyst-free inverse electron-demand Diels–Alder (IEDDA) cycloaddition with tetrazines, and an NHS ester for efficient coupling to primary amines. The PEG4 spacer specifically enhances solubility, flexibility, and reduces steric hindrance, enabling high-yield bioconjugation in both aqueous and organic systems.
Key Features and Benefits
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TCO Group – Enables ultra-fast, catalyst-free tetrazine click chemistry for bioorthogonal labeling.
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NHS Ester – Reacts efficiently with primary amines on proteins, peptides, and other biomolecules.
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PEG4 Spacer – Improves aqueous solubility and accessibility of reactive groups while minimizing steric hindrance.
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Orthogonal Reactivity – Allows sequential and site-specific modifications without cross-reactivity.
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High Purity – Supplied at >95% purity for reproducible performance.
Applications
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Bioorthogonal labeling of proteins, peptides, and nucleic acids
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Antibody–drug conjugate (ADC) linker chemistry
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Site-specific modification for drug delivery systems
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Multifunctional imaging and diagnostic probe development
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid prolonged exposure to moisture to preserve NHS ester reactivity
This product combines ultra-fast tetrazine ligation via TCO with efficient amine coupling through NHS ester chemistry, connected by a PEG4 spacer for solubility and flexibility, making it especially ideal for precise, orthogonal bioconjugation.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
