TCO-PEG4-biotin
This product is a heterobifunctional reagent that combines a trans-cyclooctene (TCO) group, a PEG4 spacer, and a biotin moiety. The TCO enables ultrafast copper-free click chemistry with tetrazines, while the biotin tag binds strongly to streptavidin and avidin systems. The PEG4 spacer specifically improves solubility and reduces steric hindrance, ensuring efficient bioconjugation and accessibility of the biotin tag.
Key Features and Benefits
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TCO Group: Supports rapid, copper-free click chemistry with tetrazines via inverse electron-demand Diels–Alder (IEDDA) ligation.
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Biotin Tag: Provides strong, specific binding to streptavidin, avidin, and NeutrAvidin.
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PEG4 Spacer: Enhances solubility and minimizes steric interference during binding and conjugation.
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Dual Functionality: Combines orthogonal click chemistry with biotin–avidin affinity capture.
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High Purity: Produced to ≥95% purity for reproducible and reliable labeling.
Applications
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Bioconjugation: Couples tetrazine-functionalized biomolecules with biotin for downstream capture or detection.
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Affinity Capture: Facilitates immobilization of conjugates on streptavidin-coated beads, plates, or sensors.
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Probe Development: Builds dual-functional probes for imaging, diagnostics, and target enrichment.
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Chemical Biology: Enables selective labeling and affinity pull-down in complex systems.
Storage and Handling
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Storage: Store at –20 °C in a tightly sealed, light-protected container.
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Handling: Dissolve freshly before use; avoid repeated freeze–thaw cycles.
This product integrates rapid TCO click chemistry with biotin affinity binding, making it an especially powerful tool for affinity capture, probe development, and advanced bioconjugation strategies.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
