TCO-PEG3-NHS ester (CAS Number: 2141981-88-4) is a polyethylene glycol (PEG) derivative featuring a trans-cyclooctene (TCO) moiety and an N-hydroxysuccinimide (NHS) ester. The NHS ester reacts efficiently with primary amines, enabling the conjugation of the TCO group to proteins, antibodies, or other amine-containing molecules. The PEG3 spacer enhances water solubility and reduces steric hindrance during bioconjugation.
Chemical Properties:
- Chemical Formula: C₂₂H₃₄N₂O₉
- Molecular Weight: 470.52 g/mol
- Physical State: Colorless oil
- Purity: ≥95%
Applications:
- Bioconjugation: Facilitates the labeling of biomolecules with the TCO moiety, which can participate in rapid and selective bioorthogonal reactions.
- PROTAC Synthesis: Serves as a linker in the assembly of Proteolysis Targeting Chimeras (PROTACs) for targeted protein degradation.
- Click Chemistry: Utilized in strain-promoted azide-alkyne cycloaddition (SPAAC) reactions due to the TCO group’s reactivity.
Storage and Handling:
- Storage Conditions: Store at -20°C in a cool, dry place, protected from light and moisture.
- Safety Measures: Handle with appropriate protective equipment; consult the Safety Data Sheet (SDS) for detailed information.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
