TCO-PEG2-NHS ester
TCO-PEG2-NHS ester is a high-purity heterobifunctional PEG linker with a trans-cyclooctene (TCO) group for rapid, catalyst-free inverse electron-demand Diels–Alder (IEDDA) click chemistry with tetrazines, and an NHS ester for efficient amine coupling. The PEG2 spacer improves aqueous solubility, enhances flexibility, and reduces steric hindrance, enabling high-yield bioconjugation in both aqueous and organic systems. This orthogonal design is ideal for stepwise conjugation strategies in drug delivery, diagnostics, and biomaterials.
Key Features and Benefits
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TCO Functional Group: Reacts rapidly with tetrazines under mild, catalyst-free conditions.
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NHS Ester: Couples efficiently with primary amines to form stable amide bonds.
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PEG2 Spacer: Enhances solubility, flexibility, and accessibility of reactive sites.
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Heterobifunctional Design: Supports orthogonal conjugation strategies.
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High Purity: Supplied at >95% purity for reproducible, reliable results.
Applications
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Bioorthogonal Labeling: Attach TCO groups for fast tetrazine ligation.
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Protein and Peptide Modification: Couple to amines while reserving TCO for click chemistry.
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Antibody–Drug Conjugates (ADCs): Enable site-specific payload attachment.
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Nanoparticle Functionalization: Add TCO handles for targeted click conjugation.
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Diagnostic Probes: Create multifunctional imaging and sensing agents.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid moisture to preserve NHS ester and TCO reactivity.
TCO-PEG2-NHS ester combines rapid, catalyst-free TCO–tetrazine click chemistry with efficient amine coupling in a hydrophilic PEG2 framework, making it an especially versatile tool for orthogonal bioconjugation in drug delivery, diagnostics, and materials science.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
