TCO-PEG2-Biotin
This product is a compact heterobifunctional linker containing a trans-cyclooctene (TCO) group, a short PEG2 spacer, and a biotin moiety. The TCO enables rapid copper-free click chemistry with tetrazines, while the biotin tag binds strongly to streptavidin and avidin systems. The PEG2 spacer specifically improves solubility and accessibility, ensuring efficient conjugation in biochemical workflows.
Key Features and Benefits
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TCO Group: Provides ultrafast copper-free click ligation with tetrazines.
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Biotin Tag: Ensures strong binding to streptavidin, avidin, and NeutrAvidin.
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PEG2 Spacer: Enhances solubility and reduces steric interference.
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Dual Reactivity: Combines click chemistry with biotin affinity capture.
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High Purity: Produced to ≥95% purity for reliable results.
Applications
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Bioconjugation: Couples tetrazine-modified biomolecules with biotin for capture or detection.
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Affinity Capture: Immobilizes conjugates on streptavidin-coated beads, plates, or biosensors.
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Probe Development: Builds dual-functional probes for imaging and diagnostics.
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Chemical Biology: Enables selective labeling and enrichment in complex samples.
Storage and Handling
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Storage: Store at –20 °C in a sealed, light-protected container.
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Handling: Use freshly prepared solutions; avoid repeated freeze–thaw cycles.
This product integrates fast click chemistry with biotin–streptavidin affinity, specifically offering a compact tool for labeling, capture, and probe development.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
