t-Boc-N-Amido-PEG5-Azide

t-Boc-N-Amido-PEG5-Azide

t-Boc-N-Amido-PEG5-Azide is a high-purity, heterobifunctional PEG linker containing a Boc-protected amine and a terminal azide group. Moreover, the PEG5 spacer increases hydrophilicity, reduces steric hindrance, and provides optimal flexibility for a wide range of bioorthogonal conjugation and multistep bioconjugation strategies. This reagent is especially valuable in applications requiring click chemistry with controlled, sequential reactivity.

Key Features and Benefits:

• Azide Functional Group: Enables efficient reaction with alkynes through copper-catalyzed (CuAAC) or copper-free strain-promoted click chemistry (SPAAC).

• Boc-Protected Amine: Allows selective deprotection under mild acidic conditions for downstream amide bond formation or other functionalization steps.

• PEG5 Spacer: Enhances solubility, increases molecular flexibility, and reduces nonspecific binding.

• Orthogonal Reactivity: Supports stepwise conjugation for building complex, multifunctional biomolecules.

• High Purity: Supplied at >95% purity to ensure consistent and reproducible bioconjugation results.

Applications:

• Click Chemistry Bioconjugation: Conjugate to alkyne- or DBCO-modified molecules for modular assembly.

• Stepwise Bioconjugation: Use the Boc-protected amine after click chemistry for orthogonal functionalization.

• Protein and Peptide Labeling: Introduce azide groups for site-specific tagging or imaging.

• Nanoparticle and Hydrogel Functionalization: Modify surfaces to enable bioorthogonal click-based attachment.

• Drug Delivery and Diagnostic Development: Build bifunctional linkers for targeted therapeutics and biosensor design.

Storage and Handling:

• Storage: Store at -20°C, dry, and protected from light.

• Handling: Deprotect the Boc group using 50% TFA in DCM or similar acidic conditions. Use anhydrous solvents such as DMSO or DMF to maintain azide reactivity.

t-Boc-N-Amido-PEG5-Azide offers reliable, orthogonal reactivity in a compact, hydrophilic design. Its balanced PEG5 length provides both flexibility and control, making it an excellent choice for click chemistry workflows, dual-labeling strategies, and advanced bioconjugation research.

AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.