Sulfo-Cyanine7 Tyramide is a near-infrared fluorescent reagent (excitation ~750 nm, emission ~775 nm) used in Tyramide Signal Amplification (TSA), an advanced method for enhancing fluorescence intensity in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). TSA leverages horseradish peroxidase (HRP) and hydrogen peroxide to convert labeled tyramides into reactive intermediates that covalently bind to tyrosine residues, increasing detection sensitivity by over 100-fold.
Key Features:
- Signal Amplification: Ideal for detecting low-abundance targets and reducing antibody/probe concentrations while maintaining signal intensity and minimizing background staining.
- Multiplexing: Covalent binding allows sequential rounds of labeling with multiple dyes for detecting multiple targets in one sample.
- Applications: IHC, ICC, FISH, and deep-tissue imaging in low-autofluorescence near-infrared wavelengths.
- Compatibility: Works with HRP-conjugated antibodies, streptavidin, and other molecules.
Storage and Handling:
- Store at -20°C, protected from light.
- Commonly included in TSA kits for sensitive detection.
Sulfo-Cyanine7 Tyramide is ideal for advanced multiplexed imaging and deep-tissue fluorescence detection with high sensitivity and low background.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
