Sulfo-Cyanine5 Tyramide is a far-red fluorescent reagent (excitation ~650 nm, emission ~670 nm) used in Tyramide Signal Amplification (TSA), an effective method to enhance fluorescence intensity in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). TSA relies on horseradish peroxidase (HRP) and hydrogen peroxide to convert labeled tyramides into reactive intermediates that covalently bind to tyrosine residues, amplifying signals over 100-fold compared to conventional methods.
Key Features:
- Signal Enhancement: Ideal for detecting low-abundance targets or reducing antibody/probe concentrations without loss of intensity, minimizing background staining.
- Multiplexing: Covalent binding enables sequential staining with multiple dyes for detecting multiple targets in a single sample.
- Applications: IHC, ICC, FISH, and fluorescence microscopy.
- Compatibility: Works with HRP-conjugated antibodies, streptavidin, and other molecules.
Storage and Handling:
- Store at -20°C, protected from light.
- Widely used in TSA kits for sensitive, specific detection.
Sulfo-Cyanine5 Tyramide is a robust choice for advanced fluorescence-based detection and multiplexed imaging.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
