Sulfo-Cyanine5 Streptavidin combines streptavidin with a water-soluble Sulfo-Cyanine5 fluorophore for sensitive fluorescent detection of biotinylated targets that specifically supports fluorescence imaging, flow cytometry, biotin-based assays, and molecular detection research.
Key Features and Benefits
- Sulfo-Cyanine5 Fluorescence: Provides bright far-red fluorescence for sensitive optical detection and imaging.
- Streptavidin-Biotin Binding: Uses the strong affinity of streptavidin specifically for biotin to detect biotinylated molecules and surfaces.
- Water-Soluble Fluorophore: Sulfonated cyanine chemistry specifically supports aqueous compatibility for biological research applications.
- Direct Fluorescent Detection: Combines biotin recognition and fluorescence in one conjugate, reducing the need for secondary detection reagents.
- Far-Red Detection: Supports fluorescence measurements in the far-red spectral region with compatible instrumentation.
- Multiplexing Potential: Far-red fluorescence specifically facilitates combination with spectrally distinct fluorescent labels in multicolor experiments.
- Versatile Detection Reagent: Supports detection of biotinylated proteins, antibodies, nucleic acids, probes, and other research materials.
Applications
- Biotin Detection: Enables fluorescent detection of biotinylated molecules through streptavidin-biotin binding.
- Fluorescence Imaging: Supports visualization of biotinylated targets in fluorescence microscopy and imaging studies.
- Flow Cytometry: Useful for detecting biotin-labeled targets in compatible flow cytometry workflows.
- Immunofluorescence Research: Supports fluorescent detection in assays using biotinylated antibodies or other affinity reagents.
- Biochemical Assays: Useful for fluorescence-based detection in biotin-streptavidin assay systems.
- Molecular Probe Research: Supports development of biotin-based fluorescent probes and detection strategies.
- Multiplex Assays: Suitable for multicolor experiments with compatible fluorophores and detection channels.
Storage and Handling
- Storage: Store according to the product specification in a tightly sealed container. Protect from light and excessive heat.
- Handling: Minimize prolonged light exposure to preserve fluorescence performance. Avoid conditions that may compromise protein stability.
Sulfo-Cyanine5 Streptavidin combines high-affinity biotin recognition with far-red fluorescent detection and specifically supports imaging, flow cytometry, biotin-based assays, and molecular detection research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
