Sulfo-Cyanine3.5 Amine is a hydrophilic fluorescent dye containing a reactive amine group, suitable for bioconjugation and labeling in various biochemical and imaging applications.
Key Features:
- Fluorescence Properties:
- Excitation: ~580 nm
- Emission: ~595 nm
- Bright, stable red-shifted fluorescence.
- Reactive Group:
- Primary amine for conjugation with activated esters, aldehydes, or other reactive groups.
- Water Solubility: Hydrophilic dye for aqueous labeling reactions.
Applications:
- Protein and Antibody Labeling:
- Conjugation to primary or secondary antibodies for immunoassays.
- Nucleic Acid Probes:
- Labeling DNA, RNA, or oligonucleotides for hybridization studies.
- Fluorescence Microscopy:
- Visualizing proteins, cells, or tissues with red-shifted fluorescence.
- Flow Cytometry:
- Enabling sensitive detection and quantification of labeled molecules.
- FRET Studies:
- As a donor or acceptor in fluorescence resonance energy transfer experiments.
- Live Cell Imaging:
- Tracking biomolecules in real-time under physiological conditions.
- ELISA and Immunoassays:
- Enhancing sensitivity in enzyme-linked immunosorbent assays.
- Drug Delivery Research:
- Labeling carriers for biodistribution studies.
Sulfo-Cyanine3.5 Amine is an excellent choice for a wide range of fluorescence-based detection and bioconjugation workflows.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
