RAPID DiI is an orange-red fluorescent, lipophilic tracer designed for fast and efficient cell membrane labeling. Its unsaturated hydrocarbon chains allow quicker diffusion in lipid bilayers compared to traditional DiI, making it ideal for neuronal tracing and cell tracking in both live and fixed tissues.
Key Features:
- Fast Diffusion: RAPID DiI labels membranes about 50% faster than conventional DiI, ensuring quick and efficient staining.
- Stable Integration: Its long hydrocarbon chains anchor firmly into lipid bilayers, ensuring uniform and lasting staining.
- Minimal Transfer: Stays localized within labeled cells, reducing unwanted dye transfer to neighboring cells.
Applications:
- Neuronal Tracing: Tracks neuronal connections using anterograde and retrograde tracing methods.
- Cell Membrane Labeling: Stains cell membranes for studying cell morphology and dynamics.
- Dual-Color Imaging: Pairs well with RAPID DiO for dual-color imaging, distinguishing different cell types or structures.
Handling and Storage:
- Solubility: Dissolves in ethanol, methanol, or DMSO for easy staining preparation.
- Storage: Keep at -20°C, protected from light, for long-term stability.
RAPID DiI’s fast labeling, strong membrane retention, and compatibility with various imaging techniques make it a valuable tool for research in cell biology, neuroscience, and molecular imaging.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.