Pyrene-PEG5-biotin
This product is a high-purity heterobifunctional probe combining a hydrophobic pyrene fluorophore with a biotin affinity tag through a PEG5 spacer. The pyrene moiety enables strong fluorescence for sensitive detection and monitoring, while the biotin group binds with high affinity to avidin or streptavidin for immobilization, capture, or purification. The PEG5 spacer improves solubility, reduces steric hindrance, and enhances probe accessibility in both aqueous and organic systems.
Key Features and Benefits
-
Pyrene Fluorophore: Provides strong fluorescence for detection, labeling, and imaging.
-
Biotin Tag: Enables high-affinity binding to avidin or streptavidin.
-
PEG5 Spacer: Improves solubility, flexibility, and reduces steric hindrance.
-
Heterobifunctional Design: Combines fluorescence detection with affinity capture.
-
High Purity: Supplied at >95% purity for reproducibility.
Applications
-
Fluorescent labeling and detection of biomolecules.
-
Streptavidin- or avidin-based affinity purification.
-
Bioassays and imaging experiments.
-
Development of multifunctional probes.
Storage and Handling
-
Storage: Store at –20 °C, dry, and protected from light.
-
Handling: Use anhydrous solvents (e.g., DMSO, DMF) and avoid prolonged light exposure.
This product combines bright pyrene fluorescence with strong biotin–streptavidin binding in a hydrophilic PEG5 framework, enabling versatile applications in detection, capture, and imaging.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
