MitoCLox is a mitochondria-targeted fluorescent probe designed to detect lipid peroxidation, especially cardiolipin oxidation. It uses a BDP 581/591 fluorophore linked to a triphenylphosphonium (TPP) cation, which helps it accumulate in mitochondria. When oxidation occurs, MitoCLox changes its fluorescence from red (590 nm) to green (520 nm), making it easy to measure oxidative damage.
Key Features:
- Mitochondrial Targeting: The TPP cation directs MitoCLox to mitochondria for specific detection.
- Fluorescence Shift: Its color changes from red to green upon oxidation, enabling clear, quantitative results.
- Cardiolipin Sensitivity: It reacts specifically to cardiolipin oxidation, critical for studying mitochondrial damage.
Applications:
- Oxidative Stress Studies: Tracks oxidative damage in mitochondria caused by stress, aging, or disease.
- Apoptosis Research: Helps study how lipid peroxidation triggers cell death.
- Drug Screening: Tests the effectiveness of antioxidants and other therapies targeting mitochondrial damage.
How to Use:
- Concentration: Use MitoCLox at 100-200 nM.
- Incubation: Allow 45-60 minutes for it to fully enter the cells.
- Measurement: Check for reduced red fluorescence (590 nm) and increased green fluorescence (520 nm) to detect lipid peroxidation.
MitoCLox is an essential tool for researchers studying mitochondrial health, oxidative damage, and treatments aimed at reducing lipid peroxidation.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
