DSPE-Polysarcosine-Biotin20

Key Properties

• A trifunctional amphiphilic lipid conjugate consisting of a DSPE membrane anchor, a hydrophilic polysarcosine (pSar20) spacer, and a terminal biotin group for specific recognition.
• The polysarcosine segment provides excellent antifouling (stealth) effect, high hydrophilicity, good biocompatibility, and low immunogenicity, serving as a favorable alternative to PEG.
• Biotin enables strong, specific, and stable binding to avidin/streptavidin, one of the strongest non-covalent interactions in biological systems.
• Inserted stably into lipid bilayers while exposing biotin on the nanoparticle surface for bioconjugation and capture.
• Soluble in organic solvents such as chloroform, methanol, and DMSO; dispersible in aqueous buffers to form uniform nanoparticles.
• Typically a white to off-white solid or waxy powder.
• Storage: −20 °C, desiccated, protected from light and oxidation.

Applications

• Used as a functional stealth lipid in liposomes, lipid nanoparticles (LNPs), micelles, and exosome engineering.
• Enables specific immobilization and purification of nanocarriers via streptavidin-coated surfaces or magnetic beads.
• Facilitates targeted delivery by bridging biotinylated antibodies, peptides, aptamers, or targeting ligands.
• Reduces non-specific protein adsorption and prolongs circulation time of nucleic acid (mRNA, siRNA, DNA) delivery systems.
• Applied in in vitro and in vivo imaging, biosensing, and targeted drug delivery platforms.
• Serves as a biotinylated PEG alternative to avoid accelerated blood clearance (ABC) and immune reactions related to PEG.