DOTMA
CAS No.: 104872-42-6
Full name: 1,2-Dioleyloxy-3-trimethylammonium propane chloride
Key Properties
- Cationic lipid with a quaternary ammonium head group and two unsaturated oleyl ether hydrophobic chains.
- High binding affinity to negatively charged nucleic acids (DNA, mRNA, siRNA) via electrostatic interaction to form stable lipoplexes.
- Extremely high transfection efficiency, generally superior to DOTAP and many traditional cationic lipids.
- Good membrane fusion activity, promoting efficient endosomal escape and intracellular delivery.
- Can form liposomes alone or be formulated with helper lipids such as DOPE.
- Soluble in organic solvents including chloroform, methanol, and DMSO; insoluble in water.
- Typically appears as a white to pale yellow waxy solid.
Applications
- High-efficiency in vitro transfection of plasmid DNA, siRNA, mRNA, and shRNA into various eukaryotic cells.
- Widely used in gene function research, gene overexpression, RNA interference, and reporter gene assays.
- Important component in non-viral delivery systems for gene therapy in preclinical studies.
- Used in the development of DNA and mRNA vaccines as a cationic lipid component.
- Applied in research of liposomal delivery systems for nucleic acid drugs and hydrophobic small molecules.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
