DBCO-PEG12-NHS ester
DBCO-PEG12-NHS ester is a high-purity heterobifunctional PEG linker with a dibenzocyclooctyne (DBCO) group for rapid, copper-free strain-promoted azide–alkyne cycloaddition (SPAAC) and an NHS ester for efficient amine coupling. The PEG12 spacer specifically enhances solubility, flexibility, and reduces steric hindrance, enabling high-yield conjugations in both aqueous and organic systems. This reagent is especially ideal for orthogonal bioconjugation, antibody–drug conjugates, and nanoparticle functionalization.
Key Features and Benefits
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DBCO Group: Enables fast, catalyst-free SPAAC click chemistry with azides.
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NHS Ester: Couples efficiently with primary amines to form stable amide bonds.
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PEG12 Spacer: Improves solubility, flexibility, and accessibility of reactive groups.
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Heterobifunctional Design: Supports orthogonal, site-specific modifications.
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High Purity: Supplied at >95% purity for reproducibility.
Applications
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Copper-free bioorthogonal labeling.
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Antibody–drug conjugate (ADC) synthesis.
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Protein, peptide, and ligand modification.
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Nanoparticle and surface functionalization.
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Targeted drug delivery system development.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid moisture to preserve NHS ester reactivity.
This product combines copper-free click chemistry and amine coupling in one linker, with a PEG12 spacer specifically for solubility and flexibility in high-efficiency bioconjugation.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
