DBCO-NH-PEG13-NHS ester
DBCO-NH-PEG13-NHS ester is a high-purity heterobifunctional PEG linker with a dibenzocyclooctyne (DBCO) group for copper-free strain-promoted alkyne–azide cycloaddition (SPAAC) and an NHS ester for rapid amine coupling. The PEG13 spacer provides excellent water solubility, increased flexibility, and reduced steric hindrance, enabling efficient bioconjugation in both aqueous and organic systems. This design is especially ideal for orthogonal conjugation strategies requiring sequential azide click labeling and amine coupling.
Key Features and Benefits
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DBCO Group: Enables copper-free click chemistry with azides for bioorthogonal conjugation.
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NHS Ester: Rapidly couples with primary amines under mild conditions to form stable amide bonds.
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PEG13 Spacer: Enhances solubility, flexibility, and accessibility of reactive groups; reduces aggregation.
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Heterobifunctional Design: Allows orthogonal, sequential modification strategies in a single molecule.
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High Purity: Supplied at >95% purity for consistent, reproducible performance.
Applications
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Stepwise Bioconjugation: Sequential azide click labeling and amine coupling.
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Protein and Peptide Modification: Introduce clickable DBCO groups while coupling to amine functionalities.
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Nanoparticle and Surface Functionalization: Modify amine-bearing surfaces with clickable DBCO handles.
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Drug Delivery Systems: Create multifunctional linkers for targeted payload delivery.
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Imaging and Diagnostic Probes: Prepare dual-labeled probes using orthogonal chemistries.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid prolonged moisture exposure to maintain NHS ester reactivity.
DBCO-NH-PEG13-NHS ester combines copper-free click chemistry and rapid amine coupling in a long PEG13 spacer, offering excellent solubility, flexibility, and orthogonal conjugation capability specifically for advanced bioconjugation applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
