Butyne-DOTA provides an alkyne-functionalized macrocyclic chelator for click chemistry, radiometal labeling, and bioconjugation research and specifically combines the DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) chelating framework with a butyne-derived alkyne functionality. This structure specifically supports covalent attachment to azide-functionalized molecules and coordination of compatible metal ions for molecular imaging and radiopharmaceutical research.
Key Features and Benefits
- DOTA Chelating Framework: Contains a macrocyclic tetraazacyclododecane-based chelator with nitrogen and oxygen donor atoms for coordination of suitable metal ions.
- Alkyne Functionality: Provides a reactive alkyne group specifically for conjugation with azide-functionalized molecules through compatible click chemistry reactions.
- Click Chemistry Compatibility: Supports copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) specifically for formation of stable triazole-linked conjugates.
- Bifunctional Chelator Design: Combines metal coordination and chemical conjugation capabilities for developing functionalized imaging and radiolabeling agents.
- Butyne-Derived Functional Group: Incorporates an alkyne-containing structural component specifically for introducing DOTA functionality into suitably modified molecules.
- Radiometal Coordination: Supports complexation studies with compatible radiometals, including lutetium-177, yttrium-90, and indium-111, under optimized conditions.
- Stable Triazole Linkage: Enables formation of covalent triazole bonds specifically through CuAAC reactions with suitable organic azides.
- Versatile Biomolecular Functionalization: Supports attachment of DOTA chelators to azide-modified peptides, proteins, polymers, and other research materials.
Applications
- Click Chemistry Bioconjugation: Supports CuAAC-mediated attachment of DOTA functionality to azide-containing biomolecules and synthetic compounds.
- Radiopharmaceutical Research: Enables preparation of chelator-functionalized constructs for investigational diagnostic and therapeutic radiometal labeling.
- Peptide Modification: Useful for conjugating DOTA to azide-functionalized peptides through alkyne-azide cycloaddition reactions.
- Protein Bioconjugation: Supports attachment of macrocyclic chelators to proteins containing appropriately introduced azide groups.
- Molecular Imaging Research: Enables development of metal-chelate probes for PET, SPECT, and other investigational imaging applications.
- Radiometal Labeling Studies: Supports investigation of metal incorporation, labeling efficiency, radiochemical purity, and complex stability.
- Polymer Functionalization: Useful for introducing DOTA chelators into azide-functionalized polymers, nanoparticles, and biomaterials.
- Targeted Radioligand Development: Supports preparation of chelator-functionalized targeting molecules specifically for radionuclide delivery research.
- Coordination Chemistry Research: Enables investigation of metal-binding behavior, complex formation, and physicochemical properties of DOTA conjugates.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container unless otherwise specified. Protect from moisture, excessive heat, and prolonged light exposure.
- Handling: Handle using appropriate laboratory precautions and minimize trace-metal contamination. For CuAAC conjugation, use suitable copper catalysts, ligands, and azide-functionalized reaction partners. Remove residual copper when necessary before subsequent metal complexation or biological studies.
Butyne-DOTA combines a macrocyclic DOTA chelator with an alkyne functionality for versatile click chemistry conjugation and specifically supports radiometal labeling, peptide and protein modification, molecular imaging, and development of functionalized radiopharmaceutical research compounds.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
