Biotin-SLC-NHS Ester
This product is a sulfo-long-chain biotin reagent featuring an NHS ester for rapid and efficient amine conjugation. The long-chain, sulfonated spacer enhances water solubility, reduces steric hindrance, and improves accessibility, making it especially ideal for biotinylation of proteins, antibodies, and biomolecules in aqueous systems.
Key Features and Benefits:
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Biotin Moiety: Provides strong avidin/streptavidin binding for detection and purification.
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NHS Ester: Couples efficiently with primary amines under mild conditions.
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SLC Spacer: Long-chain, sulfonated PEG-like structure improves solubility and accessibility.
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High Affinity + Solubility: Enables efficient labeling in aqueous buffers.
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High Purity: Ensures reproducibility in biotinylation workflows.
Applications:
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Protein Labeling: Biotinylates antibodies, enzymes, and other amine-containing proteins.
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Aqueous Bioconjugation: Ideal for reactions performed in physiological buffers.
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Diagnostics: Used in ELISA, Western blotting, and immunoprecipitation assays.
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Purification: Facilitates streptavidin-based capture and affinity separations.
Storage and Handling:
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Storage: Store at –20 °C in a tightly sealed, light- and moisture-protected container.
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Handling: Use freshly prepared solutions; avoid repeated freeze–thaw cycles.
This product combines high-affinity biotin binding with sulfonated spacer solubility, offering reliable and efficient protein labeling for biochemical assays and conjugation.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
