Biotin-PEG3-SS-azide
This product is a cleavable biotinylation reagent featuring a biotin tag, a PEG3 spacer, and a disulfide (SS) linkage connected to an azide group. The PEG3 spacer specifically improves solubility and flexibility, while the disulfide bond allows reductive cleavage. In addition, the terminal azide enables copper-catalyzed or copper-free click chemistry for versatile bioconjugation.
Key Features and Benefits
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Biotin Tag: Binds strongly to streptavidin, avidin, and NeutrAvidin systems for capture and detection.
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PEG3 Spacer: Enhances solubility, reduces steric hindrance, and improves accessibility of the biotin moiety.
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Disulfide Linker (SS): Provides a cleavable bond that can be reduced under mild conditions.
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Azide Group: Enables CuAAC or SPAAC click chemistry with alkyne-functionalized molecules.
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High Purity: Produced to ≥95% purity for reproducible labeling and reliable assay results.
Applications
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Cleavable Biotinylation: Labels proteins, peptides, and biomolecules with biotin while allowing controlled release.
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Affinity Capture and Release: Facilitates reversible immobilization on streptavidin-coated surfaces.
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Click Chemistry: Couples biomolecules or probes via azide–alkyne cycloaddition.
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Probe Development: Builds cleavable probes for imaging, pull-down assays, and target identification.
Storage and Handling
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Storage: Store at –20 °C in a dry, light-protected container.
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Handling: Dissolve freshly in dry DMSO or DMF; avoid repeated freeze–thaw cycles.
This product integrates a cleavable disulfide bond with a biotin tag and clickable azide handle, making it an especially powerful tool for affinity capture, reversible labeling, and advanced bioconjugation.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
