Biotin-PEG-Thiol (CAS: 2762393-22-4)
Biotin-PEG-Thiol is a versatile bioconjugation reagent combining biotin for strong avidin/streptavidin binding and a thiol group for covalent attachment. The PEG spacer enhances solubility, flexibility, and stability, making it ideal for a variety of applications.
Key Features:
- Biotin Group:
- Binds strongly and specifically to avidin or streptavidin, useful in purification and detection assays.
- Thiol Group (-SH):
- Reacts with thiol-reactive groups like maleimide or disulfides.
- Can also bind to gold or noble metal surfaces.
- PEG Spacer:
- Improves water solubility and reduces steric hindrance.
- Enhances stability and biocompatibility of conjugated molecules.
Biotin-PEG-Thiol Applications:
- Protein and Peptide Labeling:
- Biotinylates proteins, peptides, and antibodies for detection, immobilization, or purification.
- Surface Modification:
- Functionalizes nanoparticles, biosensors, and other materials with biotin groups for avidin/streptavidin binding.
- Nanotechnology:
- Creates biotinylated gold nanoparticles for targeted delivery and imaging.
- Diagnostic Assays:
- Used in assays relying on biotin-streptavidin interactions for signal amplification and detection.
Handling and Storage:
- Store at -20°C in a dry environment.
- Protect from air and moisture.
- Prepare fresh solutions for each use and avoid repeated freeze-thaw cycles.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
