Biotin-NHS Ester
This product is an amine-reactive biotinylation reagent containing an NHS ester group. It specifically enables efficient labeling of proteins, peptides, and other amine-containing molecules, offering strong biotin–streptavidin binding and high sensitivity in detection.
Key Features and Benefits
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Biotin Moiety: Provides strong, specific binding to streptavidin and avidin.
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NHS Ester: Couples efficiently with primary amines to form stable amide bonds.
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High Reactivity: Ensures fast labeling under mild conditions.
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Versatile Use: Suitable for proteins, peptides, antibodies, and small molecules.
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High Purity: Guarantees reproducibility in bioconjugation workflows.
Applications
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Bioconjugation: Labels proteins, peptides, and nucleic acids with biotin.
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Probe Development: Builds biotinylated probes for diagnostics and assays.
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Detection Assays: Supports ELISA, Western blotting, and immunoprecipitation.
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Affinity Purification: Enables capture and isolation of biotinylated molecules.
Storage and Handling
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Storage: Store at –20 °C in a sealed, light- and moisture-protected container.
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Handling: Dissolve in anhydrous DMSO or DMF; prepare fresh solutions for best results.
This product integrates amine-reactive chemistry with strong biotin affinity, specifically providing a robust reagent for biotinylation and detection workflows.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
