Biotin-Azide (CAS 1006592-62-6), also known as PEG4 carboxamide-6-Azidohexanyl Biotin, is a PEG derivative containing a biotin and an azide group. It reacts quickly with alkynes, DBCO, and BCN through Click Chemistry, forming a stable triazole linkage. The PEG spacer increases the solubility of biotin-conjugated molecules and reduces steric hindrance, improving flexibility in binding to streptavidin, avidin, or NeutrAvidin®.
Key Features:
- Reactivity:
- Reacts with alkynes, DBCO, and BCN through Click Chemistry
- Forms stable triazole linkages
- Applications:
- Biotinylates DNA, oligonucleotides, and proteins
- Used in affinity purification, detection, and imaging
- Improves solubility and binding due to the PEG spacer
Biotin-Azide offers efficient labeling, enhanced solubility, and flexibility for binding, making it ideal for a wide range of biochemical applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
