Biotin-Azide
This product is a biotin derivative specifically containing an azide group for click chemistry reactions. The azide functionality specifically enables efficient conjugation with alkyne-modified molecules, while the biotin moiety provides strong affinity for streptavidin and avidin binding.
Key Features and Benefits
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Azide Group: Reacts with alkynes via CuAAC or strain-promoted click chemistry.
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Biotin Moiety: Ensures high-affinity binding to streptavidin and avidin.
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High Reactivity: Enables fast, efficient conjugation under mild conditions.
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Versatile Utility: Suitable for labeling, detection, and purification workflows.
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High Purity: Synthesized and purified to ≥95% for reproducibility.
Applications
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Bioconjugation: Couples biotin to alkyne-modified proteins, peptides, and small molecules.
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Affinity Tagging: Facilitates streptavidin/avidin-based pull-down, detection, and imaging.
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Bioassays: Supports ELISA, Western blotting, and probe development.
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Chemical Biology: Enables construction of biotinylated probes and tracers.
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Diagnostics: Useful in biosensor and molecular detection platforms.
Storage and Handling
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Storage: Store at –20 °C in a sealed, desiccated container.
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Handling: Avoid moisture and prolonged exposure to light; prepare fresh solutions before use.
This product combines efficient azide-based click reactivity with the strong binding affinity of biotin, making it an especially essential reagent for bioconjugation, assay development, and molecular labeling.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.