Biocytin Hydrochloride (CAS 98930-70-2): Overview, Features, and Applications
Biocytin hydrochloride (CAS: 98930-70-2, ε-N-biotinyl-L-lysine) is a derivative of D-biotin containing an L-lysine group. It is widely used in molecular biology and biochemistry due to its strong binding affinity for avidin and streptavidin. Biocytin is essential for protein biotinylation, neuronal tracing, and studying enzyme kinetics.
Key Features of Biocytin Hydrochloride:
- Molecular Formula: C16H28N4O4S · HCl
- Molecular Weight: 406.95 g/mol
- High Solubility: Easily dissolves in water, making it suitable for biological use.
- Strong Binding: Binds effectively to avidin and streptavidin, enhancing detection and labeling efficiency.
Applications of Biocytin Hydrochloride:
- Biotinidase Studies:
- Used to explore biotinidase specificity and kinetics and measure biotinidase deficiency, which relates to metabolic disorders.
- Protein Biotinylation:
- Facilitates protein biotinylation using EDAC under slightly acidic conditions (pH 4-6), offering an alternative to NHS ester biotinylation.
- Neuronal Tracing:
- Effective in mapping neuronal connections and studying cell morphology in neuroanatomy.
- Cellular Uptake Studies:
- Serves as a model compound to examine uptake mechanisms in cells and tissues.
- Immunoassays and Labeling:
- Enhances sensitivity in assays by biotinylating proteins, antibodies, and enzymes for detection.
Summary
Biocytin hydrochloride (CAS: 98930-70-2) is a versatile tool for protein biotinylation, neuronal tracing, and studying biotinidase activity. Its high solubility and strong binding affinity make it valuable in various biological and biochemical applications, including protein lab
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