Biocytin, or ε-Biotinoyl-L-lysine, (CAS 576-19-2) is a compound combining biotin and lysine, widely used in neuroscience and biochemical research for its versatility in tracing and labeling applications.
Key Features:
- Molecular Formula: C₁₆H₂₈N₄O₄S
- Molecular Weight: 372.48 g/mol
- Appearance: White to off-white solid
- Solubility: Slightly soluble in water, soluble in aqueous base
- Melting Point: ~245°C (decomposes)
Applications:
- Neuroanatomical Tracing:
- Acts as a neuronal tracer for mapping pathways using anterograde, retrograde, and intracellular labeling.
- Its small size and solubility enable easy uptake by neurons.
- Biotinidase Assays:
- Used as a substrate for biotinidase to study enzyme activity and diagnose deficiencies.
- Protein Labeling:
- The biotin component allows strong binding to avidin or streptavidin, facilitating protein labeling in assays.
Biotinoyl-L-lysine is an essential tool for detailed neuronal mapping and biotin-related biochemical studies.
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