BDPep FL NHS Ester is an amine-reactive green fluorescent dye designed for peptide labeling, solid-phase peptide synthesis (SPPS), and biomolecular conjugation. It features a BODIPY-derived fluorophore engineered for improved stability under acidic conditions, along with a reactive N-hydroxysuccinimide (NHS) ester group. The NHS ester reacts with primary amines to form stable covalent amide linkages, while the fluorescent core supports molecular detection and imaging. Additionally, its compatibility with suitable TFA-containing cleavage conditions makes it useful for preparing fluorescent peptides and biomolecular probes.
Key Features and Benefits
- Green Fluorescent Dye: Provides bright green fluorescence for peptide labeling, molecular detection, and fluorescence imaging applications.
- Acid-Stable Fluorophore: Features a modified BODIPY-derived structure designed for improved stability under acidic conditions encountered during peptide synthesis.
- Reactive NHS Ester Group: Enables covalent coupling with primary amines, including accessible amino groups on peptides, proteins, and other biomolecules.
- Stable Amide Bond Formation: Supports formation of covalent amide linkages for fluorescent peptide and protein conjugation.
- Solid-Phase Peptide Synthesis Compatibility: Specifically supports fluorescent labeling of suitable resin-bound peptides before cleavage and purification.
- TFA Cleavage Compatibility: Offers improved resistance to compatible TFA-containing cleavage conditions compared with conventional acid-sensitive BODIPY derivatives.
- Protein and Peptide Labeling: Enables fluorescent modification of amine-containing biomolecules through established NHS ester coupling chemistry.
- Fluorescence Detection Compatibility: Supports analysis using fluorescence microscopes, plate readers, and other instruments equipped with appropriate excitation and emission settings.
- Bioconjugation Versatility: Additionally, supports preparation of fluorescent molecular probes for chemical biology, peptide research, and biomolecular interaction studies.
Applications
- Fluorescent Peptide Synthesis: Enables preparation of fluorescently labeled peptides through NHS ester coupling with accessible amino groups.
- Solid-Phase Peptide Labeling: Supports fluorescent modification of resin-bound peptides using compatible SPPS protocols.
- Protein Fluorescent Labeling: Facilitates covalent attachment of green fluorescent labels to proteins containing accessible primary amines.
- TFA-Compatible Peptide Probe Development: Specifically supports synthesis of fluorescent peptide probes requiring suitable acidic cleavage conditions.
- Peptide Localization Studies: Enables investigation of peptide distribution and localization using fluorescence detection techniques.
- Fluorescence Microscopy: Supports visualization of fluorescently labeled peptides and biomolecules in compatible imaging systems.
- Biomolecular Interaction Research: Facilitates investigation of peptide binding, molecular recognition, and protein interactions using fluorescence-based assays.
- Fluorescent Assay Development: Additionally, enables preparation of fluorescent probes for biochemical assays, molecular detection, and peptide activity studies.
- Chemical Biology Research: Provides an amine-reactive fluorescent building block for developing specialized peptide and protein conjugates.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container, protected from light and moisture. Additionally, minimize temperature fluctuations to preserve fluorescence stability and NHS ester reactivity.
- Handling: Use clean, dry equipment and appropriate protective gear. Prepare fresh solutions in anhydrous DMSO or DMF, minimizing exposure to moisture.
- Peptide Labeling: React the NHS ester with accessible primary amines in suitable solution-phase or solid-phase coupling conditions. Subsequently, optimize reaction time and reagent concentration for efficient fluorescent labeling.
- Purification and Analysis: Remove excess dye using chromatography or other appropriate purification methods. Evaluate labeling efficiency, fluorescence response, and conjugate purity using HPLC, LC-MS, or suitable analytical techniques.
BDPep FL NHS Ester combines green fluorescence, amine-reactive NHS ester chemistry, and improved acid stability for fluorescent peptide synthesis. Its reactive group supports covalent peptide and protein labeling, while its fluorophore design facilitates compatible TFA-based peptide cleavage workflows. Therefore, it provides a versatile labeling reagent for SPPS, fluorescent peptide probe development, biomolecular conjugation, and chemical biology research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
