APDye™ 488 Maleimide: Superior Fluorescence for Your Research
Bright, Green Fluorescence with Exceptional Stability
APDye™ 488 (Alexa Fluor® 488</a> Equivalent) offers a premium solution for your fluorescence needs. This green-fluorescent, water-soluble dye excels with its pH-insensitive emission across a range of pH 4 to pH 10, making it versatile for various experimental conditions. Ideal for use with the 488 nm laser line, APDye™ 488 delivers outstanding brightness, with a fluorescence quantum yield approaching 100%.
Reliable Conjugation with Maleimide Chemistry
Our APDye™ 488 Maleimide is specifically designed for efficient and stable conjugation to thiol-containing molecules. The maleimide group is renowned for its selectivity and effectiveness in forming stable thioether bonds with reduced thiols (sulfhydryl groups, –SH) at a pH range of 6.5 to 7.5. This results in conjugates that exhibit superior fluorescence intensity and enhanced photostability compared to those made with many other similar fluorophores.
Why Choose APDye™ 488 Maleimide?
- High Brightness: Enjoy one of the brightest green fluorescent dyes available, ensuring high visibility in your assays.
- Exceptional Quantum Yield: Achieve near-100% fluorescence quantum yield for reliable and consistent results.
- Superior Photostability: Benefit from reduced photobleaching and prolonged fluorescence during your experiments.
- Versatile Application: Perfect for conjugating with proteins, oligonucleotides, and ligands in a range of biochemical and cell biology applications.
Comparable to Leading Alternatives
APDye™ 488 Maleimide is structurally identical to the well-known Alexa Fluor® 488 C5 Maleimide, providing equivalent performance while offering a cost-effective solution for your research needs.
Applications
- Protein Labeling: Efficiently conjugate to proteins for detailed analysis and visualization.
- Oligonucleotide Labeling: Ideal for marking oligonucleotides in nucleic acid research.
- Ligand Conjugation: Perfect for tagging low molecular weight ligands in various biochemical assays.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
