Bridging the gap between rapidly eroding 2K and medium‑molecular‑weight 5K PCL‑Amine, this 3 kDa amine‑terminated poly(ε‑caprolactone) delivers a well‑balanced combination of chain crystallinity, solvent compatibility and hydrolytic degradation performance. Its terminal primary amine group is capable of forming stable amide bonds with carboxyl‑bearing peptides, protein fragments and small‑molecule ligands via EDC/NHS activation. Compared with the 2K counterpart, longer polymer chains increase crystallinity, slow hydrolysis rate and greatly improve colloidal stability after nanoprecipitation. It shifts experimental applicability beyond short‑term acute in‑vitro assays toward multi‑week cell‑culture evaluation and preliminary small‑scale biological validation, avoiding the excessive rigidity and ultra‑slow degradation of higher‑molecular‑weight PCL‑Amine materials. Researchers utilize this polymer to build cationic nanoparticle libraries and adjust surface ligand densities for intermediate‑duration biological studies.
Key Features: ‑ Intermediate‑length PCL backbone equipped with terminal primary amine functionality, adapted for EDC/NHS‑mediated amide coupling under mixed organic‑aqueous reaction environments ‑ Moderately raised crystallinity relative to 2K grade; maintains favourable solubility in DCM, THF and chloroform while producing dimension‑stable colloidal particles ‑ Intermediate hydrolytic erosion kinetics, sustaining particle morphology across multi‑week experimental cycles before gradual mass loss occurs ‑ Confers tunable positive surface charge to nanoparticles, enabling flexible modulation of cell‑particle adhesion and cellular uptake characteristics ‑ Spectroscopically validated amine end‑group conversion; controlled residual impurity levels compatible with cell‑based screening and intermediate‑term biological prototype assessment
Applications: ‑ Intermediate‑persistence amine‑functionalized micelles and nanoparticles for multi‑week drug‑release profiling and cellular‑interaction investigations ‑ EDC/NHS‑driven substrate surface modification for immobilizing carboxyl‑rich peptides in intermediate‑term cell‑phenotype assay workflows ‑ Fabrication of amide‑linked polymer‑biomolecule conjugates for mid‑duration biological observation, connecting transient 2K constructs and higher‑MW long‑residing analogues ‑ Bench‑top formulation screening linking fast‑degrading low‑MW and mechanically robust medium‑MW PCL‑Amine material series ‑ Exploratory development of cationic polymer‑coated substrates for biomimetic in‑vitro culture platforms
Handling & Storage: Store lyophilized polymer powder at −20 °C under dry inert‑gas atmosphere. Primary amine end‑groups readily react with atmospheric carbon dioxide to generate carbamate impurities, which will degrade conjugation activity. Avoid humid conditions as moisture accelerates both impurity formation and polyester backbone hydrolysis. Allow sealed sample vials to fully equilibrate inside a desiccator before opening to prevent condensed water contaminating solid samples. Prepare working solutions with anhydrous organic solvents immediately prior to experiments; long‑term storage of dissolved polymer stocks is not recommended. This product is for laboratory‑only research and is not approved for human clinical applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.