This low‑molecular‑weight amine‑terminated poly(ε‑caprolactone) carries short aliphatic polyester chains with terminal primary amine groups, designed for carbodiimide‑driven amide‑formation reactions with carboxyl‑containing biomolecules. Differing from higher‑MW PCL‑Amine variants, the 2 kDa grade features low crystallinity, excellent solubility in common organic solvents and fast hydrolytic degradation kinetics. The nucleophilic amine terminus readily couples with carboxylic‑acid‑bearing peptides, small‑molecule ligands and carboxylated substrates after EDC/NHS activation. This reactive polymer is widely adopted for fabricating rapidly eroding cationic nanoparticle prototypes and short‑term bioconjugates, well‑suited for transient in‑vitro assays and preliminary short‑term in‑vivo testing, without the extended residence behaviour of medium‑ and high‑molecular‑weight PCL‑Amine materials.
Key Features: ‑ Short‑chain low‑crystallinity PCL backbone functionalized with terminal primary amine group, optimized for EDC/NHS‑mediated amide ligation with carboxyl‑functionalized substrates ‑ Outstanding solubility in DCM, THF and chloroform, supporting straightforward nanoprecipitation and solution‑phase coupling reaction workflows ‑ Fast hydrolytic erosion performance; polymer matrix undergoes rapid mass loss over short experimental cycles with minimal persistent residual accumulation ‑ Introduces positive surface potential to assembled nanoparticles, enabling modulation of particle‑cell adhesion and internalization behaviours ‑ Spectroscopically confirmed amine end‑group fidelity; low residual catalyst content suitable for proof‑of‑concept biological laboratory screening
Applications: ‑ Fast‑degrading amine‑functionalized cationic micelles and nanoparticles for short‑term drug‑loading and cellular‑level in‑vitro investigations ‑ EDC/NHS‑assisted surface modification for immobilizing carboxyl‑rich peptides for transient cell‑interaction assay development ‑ Construction of short‑lived amide‑linked polymer‑biomolecule conjugates for acute biological evaluation, not intended for long‑term implant‑related research ‑ Exploratory ligand‑conjugation screening workflows before scaling experiments toward higher‑molecular‑weight PCL‑Amine polymer grades ‑ Bench‑scale formulation optimisation for cationic polyester nanoparticle prototypes
Handling & Storage: Preserve lyophilized polymer powder at −20 °C under dry inert‑gas atmosphere and strictly avoid humid surroundings. Primary amine groups are susceptible to reacting with ambient carbon dioxide to form carbamate impurities, which will reduce conjugation reactivity. Allow sealed vials to achieve full room‑temperature equilibration within a desiccator before opening to prevent water condensation contaminating solid samples. Prepare working solutions with anhydrous organic solvents right before experiments; prolonged storage of dissolved polymer aliquots is discouraged. This product is for laboratory‑research‑only usage and is not validated for human clinical applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.