Sulfo-Cyanine5 Dye: Spectral Properties and Applications
Sulfo-Cyanine5 (Sulfo-Cy5) is a water-soluble, far-red fluorescent dye commonly used in biological and biomedical research. Its sulfonate groups enhance water solubility, making it ideal for labeling proteins, nucleic acids, and other biomolecules in aqueous environments. With strong NIR fluorescence, high photostability, and minimal background interference, Sulfo-Cy5 is widely used in imaging and bioconjugation applications.
Spectral Properties of Sulfo-Cyanine5 Dye:
Absorption Maximum: ~646 nm
Emission Maximum: ~662 nm
Stokes Shift: ~16 nm
High Quantum Yield: Delivers bright and efficient far-red fluorescence.
Enhanced Solubility: Sulfonate groups improve performance in water-based assays.
Applications of Sulfo-Cyanine5 Dye:
Fluorescence Microscopy: Offers high-contrast imaging of cellular structures.
Flow Cytometry: Provides distinct signals for precise cell sorting and analysis.
In Vivo Imaging: Ideal for deep tissue imaging with low background interference.
Bioconjugation: Efficiently labels proteins, antibodies, and nucleic acids for various assays.
Molecular Probes: Used in bioanalytical assays to study biomolecular interactions.
Sulfo-Cy5’s bright far-red fluorescence, water solubility, and excellent photostability make it a versatile and reliable dye for advanced research and diagnostic applications.