OH-PEG-SC, MW 2K provides a heterobifunctional polyethylene glycol derivative for bioconjugation, protein PEGylation, and biomaterials research and specifically combines a 2 kDa hydrophilic PEG chain with a terminal hydroxyl (OH) group and an activated succinimidyl carbonate (SC) group. This structure supports covalent coupling with primary amines while retaining a hydroxyl functionality for subsequent chemical modification.
Key Features and Benefits
- 2 kDa PEG Chain: Features a hydrophilic polyethylene glycol backbone with a nominal molecular weight of 2,000 Da for bioconjugation and polymer modification.
- Heterobifunctional Structure: Combines hydroxyl and succinimidyl carbonate groups at opposite PEG termini for especially versatile chemical functionalization.
- Activated Succinimidyl Carbonate: Provides an amine-reactive carbonate functionality specifically for covalent conjugation with primary amines.
- Stable Carbamate Bond Formation: Reacts with suitable amino groups to form covalent carbamate linkages between PEG and conjugated molecules.
- Terminal Hydroxyl Functionality: Retains an OH group for further derivatization, polymer coupling, or surface modification.
- Hydrophilic PEG Backbone: Supports investigation of aqueous compatibility, molecular hydration, and solubility of PEG-modified compounds.
- Selective Amine Conjugation: Enables attachment to accessible lysine residues, N-terminal amino groups, and other primary amines under optimized conditions.
- Short PEG Chain: Provides a 2 kDa PEG spacer specifically for investigating molecular flexibility, steric effects, and surface hydration while introducing less polymer mass than higher-molecular-weight alternatives.
- Versatile Chemical Modification: Supports preparation of PEGylated proteins, peptides, polymers, and functionalized biomaterials.
Applications
- Protein PEGylation: Supports covalent attachment of 2 kDa PEG chains to accessible protein amino groups for stability and solubility studies.
- Peptide Modification: Enables PEGylation of peptides containing suitable primary amines for investigating molecular properties and conjugation efficiency.
- Bioconjugation Research: Supports succinimidyl carbonate-mediated coupling of PEG to amine-functionalized biomolecules and synthetic compounds.
- Drug Delivery Research: Enables preparation of PEG-modified molecular constructs for investigating formulation properties and delivery systems.
- Biomaterial Functionalization: Supports modification of amine-containing hydrogels, polymers, and biomaterial surfaces with hydrophilic PEG chains.
- Surface Modification: Useful for introducing PEG functionality onto amine-containing surfaces to investigate hydration and nonspecific adsorption.
- Hydroxyl Functionalization: Enables further chemical modification of the remaining terminal hydroxyl group after amine conjugation.
- Polymer Conjugate Development: Supports preparation of heterobifunctional PEG conjugates for advanced polymer and materials research.
- Protein Stability Studies: Enables investigation of PEGylation effects on protein aggregation, molecular interactions, and physicochemical stability.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container under an inert atmosphere when possible, unless otherwise specified. Protect from moisture and excessive heat to minimize hydrolysis of the activated carbonate group.
- Handling: Handle under dry conditions and prepare solutions immediately before use. For amine conjugation, dissolve the reagent in a compatible dry solvent or suitable amine-free reaction buffer. Phosphate-based buffers around pH 7.0–8.5 may be appropriate, depending on the target molecule. Avoid Tris and other primary amine-containing buffers that compete with the conjugation reaction. Minimize prolonged exposure to aqueous solutions to preserve activated carbonate reactivity.
OH-PEG-SC, MW 2K combines an amine-reactive succinimidyl carbonate group with a terminal hydroxyl functionality for versatile PEG conjugation and specifically supports protein and peptide PEGylation, biomaterial functionalization, surface modification, drug delivery research, and development of advanced polymer conjugates.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
