Fluorescent fuel is also called fluorescent probe. She has the advantages of fast detection speed, good reproducibility, less sample volume, and no radiation. Therefore, researchers will widely use this fluorescent label.
Most fluorescent dyes are not selective and are fluorescent dyes immobilized on biologically active carriers. It can take advantage of the selectivity between biologically active substances (antigen-antibody/biotin-streptavidin) and modify functional groups with appropriate dye molecules, and then achieve targeted labeling of target substances through interactions between functional groups. The following are a few common types of reactive fluorescent dyes.
1.Succinimidyl ester/NHS grease
Succinimide is also known as butyrimide or succinimide. This is a colorless needle-like crystal or flake solid with light brown luster, sweet taste. Used to label-free amino acids (-NH2) on antibodies, proteins, peptides, amine-modified oligonucleotides, and other biomolecules.
2.Maleimide
Maleimide is an organic substance with a chemical formula of C4H3NO2, flake crystals, easy to sublime, and soluble in water, ether, and ethanol. Used for antibody labeling, sulfhydryl groups on proteins and peptides.
3.Azide
Azide compounds are inhibitors of the electron transport system. It can form coordination compounds with cytochromes to prevent the reduction of cytochrome oxidase oxidized a3 component. Tag vinyl by clicking the chemistry method.
4.Alkynes
Alkynes are the general term for hydrocarbons containing carbon-carbon triple bonds in their molecules. Mark the azide by clicking the chemistry method.
5.Carboxylic acid
The compound with a carboxyl group (-COOH) in the molecule is called carboxylic acid. After being preactivated with carbodiimide, it is used to label amines or to Steglich esterification of alcohols.
Carbonyl Reactive
Aminooxy PEG, Hydrazide PEG
6.Amino
Fluorescent dyes with free amino acids in amino groups It is used for coupling with various electrophilic compounds. Such as activated lipids and epoxides.
Reference:
http://www.bio-review.com/nhs-esters/