AFDye-488-TFP Ester (Alexa Fluor-488-TFP Ester Equivalent) CAS: 2133404-55-2 is a bright, green fluorescent dye equivalent to Alexa Fluor 488. It features a tetrafluorophenyl (TFP) ester that reacts quickly with primary amines in proteins, peptides, and antibodies. Ideal for bioconjugation, it offers strong fluorescence for various imaging and detection applications.
Key Feakeywrodstures:
- Bright Green Fluorescence: Emits at ~495 nm (excitation) and ~519 nm (emission) for clear detection.
- TFP Ester: Efficiently binds to primary amines, forming stable amide bonds.
- High Photostability: Ensures consistent fluorescence during long imaging sessions.
Applications:
- Protein and Peptide Labeling: Tags biomolecules for accurate fluorescence-based assays.
- Fluorescence Microscopy: Delivers high-resolution imaging of cells and tissues.
- Flow Cytometry: Facilitates the detection of fluorescently labeled cells.
- Bioconjugation: Enables precise fluorescent labeling in proteins and antibodies.
Advantages:
- Strong Fluorescence: Provides high sensitivity for detection.
- Durable Bonds: Ensures long-lasting fluorescent labels.
- Versatile: Works across various biological and biochemical applications.
AFDye-488-TFP Ester (CAS: 2133404-55-2) is perfect for researchers needing fast, efficient, and bright fluorescent labeling in microscopy, flow cytometry, and other detection methods.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.