α‑Hydroxyl‑ω‑Rhodamine B‑poly(D,L‑lactide) (PDLLA‑RhB, MW 2K) is linear, mono‑Rhodamine B‑terminated fully amorphous biodegradable aliphatic polyester. D‑lactide and L‑lactide repeat units are randomly distributed along polymer backbone, disrupting stereoregularity and eliminating crystalline domains. As low‑molecular‑weight fluorescent PDLLA derivative, this 2 kDa grade exhibits low hydrophobicity and rapid, homogeneous hydrolytic degradation rate. One polymer terminus is covalently conjugated with Rhodamine B red‑emitting fluorophore (excitation ~550 nm, emission ~580 nm), the opposite terminus retains inert hydroxyl (‑OH) group. Rhodamine B possesses high fluorescence quantum yield and moderate photostability, enabling red‑channel fluorescence tracing, qualitative and quantitative detection of PDLLA‑based materials via fluorescence microscopy, confocal imaging and flow cytometry. It functions as low‑molecular‑weight fluorescent macromonomer for nanoparticle labelling, cell internalization study, short‑term biodegradation and in‑vitro biodistribution biomedical research.
Key Features
- MW: 2000 Da, linear α‑hydroxyl‑ω‑Rhodamine B‑terminated poly(D,L‑lactide)
- Fully amorphous non‑crystalline structure, random D/L‑lactide sequence, no melting point
- Low hydrophobicity, rapid and uniform hydrolytic degradation versus higher‑MW PDLLA‑RhB variants
- Excellent biocompatibility; degradation produces non‑toxic lactic‑acid metabolites
- Single terminal Rhodamine B fluorophore; Ex/Em ≈550 nm / 580 nm; opposite polymer end is inert hydroxyl ‑OH
- Narrow molecular‑weight distribution, stable batch‑to‑batch reproducibility; controlled dye‑labelling ratio, minimal self‑quenching
- Soluble in DCM, CHCl₃, THF, DMF, acetone; insoluble in water
- Rhodamine B susceptible to photobleaching; strictly avoid prolonged light exposure, high‑temperature and strong acidic‑alkaline environments
Applications
- Fluorescent tracer building block for preparation of RhB‑labelled low‑molecular‑weight PDLLA nanoparticles, micelles and polymer coatings
- In‑vitro cellular uptake, endosomal trafficking visualization under confocal laser scanning microscopy and flow cytometry
- Short‑term in‑vitro degradation monitoring and in‑vitro material‑cell interaction evaluation
- Research‑grade fluorescent raw material for red‑channel imaging of fast‑degrading biodegradable drug‑delivery systems
Handling & Storage
- Store at −20 °C under dry inert nitrogen or argon atmosphere; strictly moisture‑proof and full light‑protection
- Keep hermetically sealed; moisture, heat and illumination trigger ester hydrolysis and Rhodamine B photobleaching; avoid repeated freeze‑thaw cycles
- For research‑only use; not intended for clinical or diagnostic applications
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.