α‑Hydroxyl‑ω‑Rhodamine B‑poly(D,L‑lactide) (PDLLA‑RhB, MW 20K) is linear, mono‑Rhodamine B‑terminated fully amorphous biodegradable aliphatic polyester. D‑lactide and L‑lactide repeat units are randomly distributed along polymer backbone, disrupting stereoregularity and eliminating crystalline domains. Within PDLLA‑RhB product series, this 20 kDa grade delivers the highest hydrophobicity and exhibits ultra‑slow homogeneous hydrolytic degradation. One polymer terminus is covalently conjugated with Rhodamine B red‑emitting fluorophore (excitation ~550 nm, emission ~580 nm), the opposite terminus retains inert hydroxyl (‑OH) group. Rhodamine B possesses high fluorescence quantum yield and moderate photostability, enabling red‑channel fluorescence tracing, qualitative and quantitative detection of PDLLA‑based materials via fluorescence microscopy, confocal laser scanning microscopy and flow cytometry. It acts as fluorescent macromonomer for nanoparticle labelling, cell internalization assay, ultra‑long‑term biodegradation monitoring and in‑vitro & in‑vivo biodistribution biomedical researchNanosoft P….
Key Features
- MW: 20000 Da, linear α‑hydroxyl‑ω‑Rhodamine B‑terminated poly(D,L‑lactide)
- Fully amorphous non‑crystalline structure, random D/L‑lactide sequence, no melting point
- Highest hydrophobicity in PDLLA‑RhB portfolio, ultra‑slow and uniform hydrolytic degradation
- Excellent biocompatibility; degradation produces non‑toxic lactic‑acid metabolites
- Single terminal Rhodamine B fluorophore; Ex/Em ≈550 nm / 580 nm; opposite polymer end is inert hydroxyl ‑OH
- Narrow molecular‑weight distribution, stable batch‑to‑batch reproducibility; controlled dye‑labelling ratio, reduced self‑quenching
- Soluble in DCM, CHCl₃, THF, DMF, hot acetone; insoluble in water
- Rhodamine B susceptible to photobleaching; strictly avoid prolonged light exposure, high‑temperature and strong acidic‑alkaline environments
Applications
- Fluorescent tracer building block for fabrication of RhB‑labelled PDLLA nanoparticles, microspheres and dense polymer films for ultra‑long‑acting delivery systems
- In‑vitro cellular uptake and endosomal trafficking visualization under confocal laser scanning microscopy and flow cytometry
- Ultra‑long‑term in‑vitro degradation monitoring and material‑cell interaction evaluation
- Research‑grade fluorescent raw material for red‑channel imaging of ultra‑long‑acting biodegradable sustained‑release drug‑delivery carriers
Handling & Storage
- Store at −20 °C under dry inert nitrogen or argon atmosphere; strictly moisture‑proof and full light‑protection
- Keep hermetically sealed; moisture, heat and illumination trigger ester hydrolysis and Rhodamine B photobleaching; avoid repeated freeze‑thaw cycles
- For research‑only use; not intended for clinical or diagnostic applications
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.