Marking the entry‑level medium‑molecular‑weight grade of amine‑terminated poly(ε‑caprolactone), this 5 kDa polymer exhibits enhanced chain entanglement and higher crystallinity compared with 2K and 3K low‑molecular‑weight analogues. The terminal primary amine moiety can form stable amide bonds with carboxyl‑containing proteins, aptamers and targeting ligands through EDC/NHS activation. Extended polymer chains decelerate hydrolytic degradation and significantly strengthen colloidal stability after nanoprecipitation. It moves experimental usage past short‑lived acute assays toward multi‑week cell‑culture studies and early‑stage subcutaneous in‑vivo validation. Formulation researchers select this grade to construct cationic functional particulate carriers that sustain morphological integrity throughout biological evaluation, without the ultra‑slow erosion behaviour shown by 10K and higher‑molecular‑weight PCL‑Amine variants.
Key Features: ‑ Medium‑length PCL backbone terminated with primary amine group; tolerates brief aqueous exposure during nanoparticle preparation without sharp drop of coupling reactivity ‑ Distinct chain‑entanglement effect improves particle dimensional stability, greatly reducing premature particle disassembly versus 2K / 3K low‑MW counterparts ‑ Moderate hydrolytic erosion kinetics; maintains particle and scaffold morphology across multi‑week experimental cycles before gradual bulk mass loss proceeds ‑ Generates tunable positive surface potential on nanoparticles, enabling flexible modulation of cell‑particle interaction and cellular‑uptake performance ‑ Spectroscopically verified amine end‑group conversion; low residual monomer and catalyst content compatible with cell‑level assays and preliminary subcutaneous small‑animal trials
Applications: ‑ Amine‑functionalized cationic nanoparticles and micelles for multi‑week drug‑release profiling and cellular‑interaction research after EDC/NHS‑assisted ligand conjugation ‑ EDC/NHS‑mediated surface bio‑modification of electrospun thin‑film scaffolds for building subcutaneous biomimetic in‑vitro tissue‑culture models ‑ Synthesis of amide‑linked polymer‑biomolecule conjugates for preliminary passive‑targeting prototype evaluation within subcutaneous small‑animal settings ‑ Formulation development bridging fast‑eroding low‑MW PCL‑Amine and mechanically robust high‑molecular‑weight amine‑terminated PCL polymers ‑ Laboratory screening of cationic particulate carriers before transitioning to implant‑oriented high‑MW polymer formulations
Handling & Storage: Store lyophilized polymer powder at −20 °C under dry inert‑gas protection. Terminal primary amine groups are prone to react with ambient carbon dioxide to form carbamate impurities, impairing bioconjugation efficiency. Moisture will further accelerate polyester backbone hydrolysis and impurity generation. Equilibrate sealed vials fully to room temperature inside a desiccator prior to opening, preventing water‑vapour‑triggered sample contamination. Prepare working solutions using anhydrous organic solvents right before experimental operations; long‑term storage of dissolved polymer stocks is discouraged. Intended exclusively for laboratory research; not authorised for human clinical usage.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.